Drug loading of naringin nanofibrous membrane influences osteoblast proliferation and differentiation

doi:10.3969/j.issn.2095-4344.2013.25.003

Abstract

Abstract:

BACKGROUND: Guided bone regeneration membrane just plays a mechanical barrier and isolation effect, but cannot exert an active induction role. Numerous studies have shown that naringin can promote the proliferation and differentiation of a variety of osteoblast-like cells.
OBJECTIVE: To prepare the guided bone regeneration membranes carrying naringin and to design the optimal drug loading of the membrane.
METHODS: Nanofibrous membranes containing different contents of naringin (0, 0.1, 1, 10, 100 g/L) were prepared by electrospinning. The morphologies of the membranes were observed by scanning electron microscope. MC3T3-E1 osteoblasts were seeded on the membranes. The morphologies of the cell-membrane composites were observed by scanning electron microscope. The cell proliferation was tested by methyl thiazolyl tetrazolium assay and the cell differentiation was evaluated by alkaline phosphatase activity.
RESULTS AND CONCLUSION: Smooth and uniform nanofibers were obtained by electrospinning with drug loading of 0.1, 1, 10 g/L naringin. There were no significant differences among the diameters of these three kinds of nanofibers. Many beads were observed in the nanofibrous membrane containing 100 g/L naringin. The results of methyl thiazolyl tetrazolium assay and alkaline phosphatase activity showed, as compared with the control group (containing 0 g/L naringin), the nanofibrous membrane containing 10 g/L naringin significantly promoted cell proliferation and differentiation (P < 0.05), while that containing 1 and 10 g/L naringin significantly increased alkaline phosphatase activity in osteoblasts (P < 0.05). The electrospinning naringin nanofibrous membrane with the optimal drug loading of 10 g/L promoted osteoblast proliferation and differentiation most significantly. Therefore, this new type of guided bone regeneration membranes will have a wonderful prospect in repair of bone defect under the state of osteoporosis.

Key words: biomaterials, tissue-engineered bone materials, nanomaterials, electrospinning, naringin, nanofibrous membranes, drug-loading, osteoblasts, proliferation, differentiation, provincial grants-supported paper

CLC Number:

R318

Ji Yan, Wang Lu, You Tao, Wu Xiao-hong. Drug loading of naringin nanofibrous membrane influences osteoblast proliferation and differentiation [J]. Chinese Journal of Tissue Engineering Research, 2013, 17(25): 4577-4584.

Figures/Tables 4

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